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20 micron counting chamber slide leja products  (Leja Products)

 
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    Leja Products 20 micron counting chamber slide leja products
    20 Micron Counting Chamber Slide Leja Products, supplied by Leja Products, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/count+chamber+slides+of+20/chamber+slide+leja+products/pmc11042374__media___1-122-11-15
    Average 90 stars, based on 1 article reviews
    20 micron counting chamber slide leja products - by Bioz Stars, 2026-09
    90/100 stars

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    Transferring:

    Article Title: Identification, Characterization and Synthesis of Walterospermin, a Sperm Motility Activator from the Egyptian Black Snake Walterinnesia aegyptia Venom
    Article Snippet: .. The following materials originate from: ZipTip C18 pipette tips (Millipore, Molsheim, France), sperm motility (M2 medium Sigma Aldrich, Saint-Quentin-Fallavier, France), count chamber slides of 20 and 100 μm depth (Leja Products, Nieuw-Vennep, Netherlands), chymotrypsin and α-cyano-4-hydroxycinnamic acid (CHCA) mass spectrometry matrix (Sigma-Aldrich, Saint-Quentin Fallavier, France), mass spectrometry grade trypsin gold and sequencing grade V8 protease (Promega, Charbonnières-les-Bains, France). .. Two mg of crude W. aegyptia venom was fractionated into 24 fractions with an XBridge TM BEH 130 analytical RP-HPLC C18 column (4.6 mm × 250 mm column of 3.5 μm) using an Agilent 1260 HPLC (Agilent Technologies, Les Ulis, France).

    Mass Spectrometry:

    Article Title: Identification, Characterization and Synthesis of Walterospermin, a Sperm Motility Activator from the Egyptian Black Snake Walterinnesia aegyptia Venom
    Article Snippet: .. The following materials originate from: ZipTip C18 pipette tips (Millipore, Molsheim, France), sperm motility (M2 medium Sigma Aldrich, Saint-Quentin-Fallavier, France), count chamber slides of 20 and 100 μm depth (Leja Products, Nieuw-Vennep, Netherlands), chymotrypsin and α-cyano-4-hydroxycinnamic acid (CHCA) mass spectrometry matrix (Sigma-Aldrich, Saint-Quentin Fallavier, France), mass spectrometry grade trypsin gold and sequencing grade V8 protease (Promega, Charbonnières-les-Bains, France). .. Two mg of crude W. aegyptia venom was fractionated into 24 fractions with an XBridge TM BEH 130 analytical RP-HPLC C18 column (4.6 mm × 250 mm column of 3.5 μm) using an Agilent 1260 HPLC (Agilent Technologies, Les Ulis, France).

    Sequencing:

    Article Title: Identification, Characterization and Synthesis of Walterospermin, a Sperm Motility Activator from the Egyptian Black Snake Walterinnesia aegyptia Venom
    Article Snippet: .. The following materials originate from: ZipTip C18 pipette tips (Millipore, Molsheim, France), sperm motility (M2 medium Sigma Aldrich, Saint-Quentin-Fallavier, France), count chamber slides of 20 and 100 μm depth (Leja Products, Nieuw-Vennep, Netherlands), chymotrypsin and α-cyano-4-hydroxycinnamic acid (CHCA) mass spectrometry matrix (Sigma-Aldrich, Saint-Quentin Fallavier, France), mass spectrometry grade trypsin gold and sequencing grade V8 protease (Promega, Charbonnières-les-Bains, France). .. Two mg of crude W. aegyptia venom was fractionated into 24 fractions with an XBridge TM BEH 130 analytical RP-HPLC C18 column (4.6 mm × 250 mm column of 3.5 μm) using an Agilent 1260 HPLC (Agilent Technologies, Les Ulis, France).



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    Image Search Results


    Overview of control tower with fluid management system and the eFlow chamber on a microscope stage. The pre-diluted sperm sample is automatically transferred from the sample container to the reusable eFlow chamber under the microscope objective. The microscope has a camera, which is connected to a PC with the ANDROVISION CASA software.

    Journal: Scientific Reports

    Article Title: Comparison of NUCLEOCOUNTER, ANDROVISION with Leja chambers and the newly developed ANDROVISION eFlow for sperm concentration analysis in boars

    doi: 10.1038/s41598-022-16280-6

    Figure Lengend Snippet: Overview of control tower with fluid management system and the eFlow chamber on a microscope stage. The pre-diluted sperm sample is automatically transferred from the sample container to the reusable eFlow chamber under the microscope objective. The microscope has a camera, which is connected to a PC with the ANDROVISION CASA software.

    Article Snippet: To compare the concentration measurements of different methods, 109 diluted boar semen samples from a commercial AI center were measured for sperm concentration with the NUCLEOCOUNTER, ANDROVISION eFlow and ANDROVISION with disposable counting chambers (Leja, 4-chamber counting slides; 20 μm nominal chamber depth, Leja Products B.V., Nieuw-Vennep, The Netherlands) in a split sample design.

    Techniques: Control, Microscopy, Software

    Results (mean, SD, minimum and maximum) of sperm concentration measurement with two methods (NUCLEOCOUNTER, ANDROVISION eFlow) from 185 raw boar ejaculates. The mean values for sperm concentration did not differ significantly (paired Student’s t-test; P = 0.25).

    Journal: Scientific Reports

    Article Title: Comparison of NUCLEOCOUNTER, ANDROVISION with Leja chambers and the newly developed ANDROVISION eFlow for sperm concentration analysis in boars

    doi: 10.1038/s41598-022-16280-6

    Figure Lengend Snippet: Results (mean, SD, minimum and maximum) of sperm concentration measurement with two methods (NUCLEOCOUNTER, ANDROVISION eFlow) from 185 raw boar ejaculates. The mean values for sperm concentration did not differ significantly (paired Student’s t-test; P = 0.25).

    Article Snippet: To compare the concentration measurements of different methods, 109 diluted boar semen samples from a commercial AI center were measured for sperm concentration with the NUCLEOCOUNTER, ANDROVISION eFlow and ANDROVISION with disposable counting chambers (Leja, 4-chamber counting slides; 20 μm nominal chamber depth, Leja Products B.V., Nieuw-Vennep, The Netherlands) in a split sample design.

    Techniques: Concentration Assay

    Method comparison of NUCLEOCOUNTER and eFlow sperm concentration of 185 raw boar ejaculates as measured in split samples with the NUCLEOCOUNTER as reference method and ANDROVISION eFlow. The Concordance Correlation Coefficient (CCC) of the method comparison was 0.955.

    Journal: Scientific Reports

    Article Title: Comparison of NUCLEOCOUNTER, ANDROVISION with Leja chambers and the newly developed ANDROVISION eFlow for sperm concentration analysis in boars

    doi: 10.1038/s41598-022-16280-6

    Figure Lengend Snippet: Method comparison of NUCLEOCOUNTER and eFlow sperm concentration of 185 raw boar ejaculates as measured in split samples with the NUCLEOCOUNTER as reference method and ANDROVISION eFlow. The Concordance Correlation Coefficient (CCC) of the method comparison was 0.955.

    Article Snippet: To compare the concentration measurements of different methods, 109 diluted boar semen samples from a commercial AI center were measured for sperm concentration with the NUCLEOCOUNTER, ANDROVISION eFlow and ANDROVISION with disposable counting chambers (Leja, 4-chamber counting slides; 20 μm nominal chamber depth, Leja Products B.V., Nieuw-Vennep, The Netherlands) in a split sample design.

    Techniques: Comparison, Concentration Assay

    Bland–Altman plot of split-sample measurements of sperm concentration with ANDROVISION eFlow and NUCLEOCOUNTER (reference method). 95.1% of all measurements lie within mean ± 1.96 SD. The confidence interval (shaded area) of the mean of the absolute differences (± 6.13 × 10 6 , CI = − 2.51 × 10 6 –9.75 × 10 6 ) includes the x-axis.

    Journal: Scientific Reports

    Article Title: Comparison of NUCLEOCOUNTER, ANDROVISION with Leja chambers and the newly developed ANDROVISION eFlow for sperm concentration analysis in boars

    doi: 10.1038/s41598-022-16280-6

    Figure Lengend Snippet: Bland–Altman plot of split-sample measurements of sperm concentration with ANDROVISION eFlow and NUCLEOCOUNTER (reference method). 95.1% of all measurements lie within mean ± 1.96 SD. The confidence interval (shaded area) of the mean of the absolute differences (± 6.13 × 10 6 , CI = − 2.51 × 10 6 –9.75 × 10 6 ) includes the x-axis.

    Article Snippet: To compare the concentration measurements of different methods, 109 diluted boar semen samples from a commercial AI center were measured for sperm concentration with the NUCLEOCOUNTER, ANDROVISION eFlow and ANDROVISION with disposable counting chambers (Leja, 4-chamber counting slides; 20 μm nominal chamber depth, Leja Products B.V., Nieuw-Vennep, The Netherlands) in a split sample design.

    Techniques: Concentration Assay

    Plots of coefficient of variation (CV) of sperm concentration double measurements with NUCLEOCOUNTER, ANDROVISION eFlow and Leja chamber, respectively ( a,b,c P < 0.0001).

    Journal: Scientific Reports

    Article Title: Comparison of NUCLEOCOUNTER, ANDROVISION with Leja chambers and the newly developed ANDROVISION eFlow for sperm concentration analysis in boars

    doi: 10.1038/s41598-022-16280-6

    Figure Lengend Snippet: Plots of coefficient of variation (CV) of sperm concentration double measurements with NUCLEOCOUNTER, ANDROVISION eFlow and Leja chamber, respectively ( a,b,c P < 0.0001).

    Article Snippet: To compare the concentration measurements of different methods, 109 diluted boar semen samples from a commercial AI center were measured for sperm concentration with the NUCLEOCOUNTER, ANDROVISION eFlow and ANDROVISION with disposable counting chambers (Leja, 4-chamber counting slides; 20 μm nominal chamber depth, Leja Products B.V., Nieuw-Vennep, The Netherlands) in a split sample design.

    Techniques: Concentration Assay

    Comparison of boar sperm concentrations (diluted semen) measurements methods. ANDROVISION eFlow and disposable counting chambers (Leja) were tested against the NUCLEOCOUNTER (NC), respectively. The linear regressions are defined as y = 1.06 + 0.94 × for ANDROVISION eFlow and y = − 3.6 + 1.04 × for ANDROVISION with Leja chambers.

    Journal: Scientific Reports

    Article Title: Comparison of NUCLEOCOUNTER, ANDROVISION with Leja chambers and the newly developed ANDROVISION eFlow for sperm concentration analysis in boars

    doi: 10.1038/s41598-022-16280-6

    Figure Lengend Snippet: Comparison of boar sperm concentrations (diluted semen) measurements methods. ANDROVISION eFlow and disposable counting chambers (Leja) were tested against the NUCLEOCOUNTER (NC), respectively. The linear regressions are defined as y = 1.06 + 0.94 × for ANDROVISION eFlow and y = − 3.6 + 1.04 × for ANDROVISION with Leja chambers.

    Article Snippet: To compare the concentration measurements of different methods, 109 diluted boar semen samples from a commercial AI center were measured for sperm concentration with the NUCLEOCOUNTER, ANDROVISION eFlow and ANDROVISION with disposable counting chambers (Leja, 4-chamber counting slides; 20 μm nominal chamber depth, Leja Products B.V., Nieuw-Vennep, The Netherlands) in a split sample design.

    Techniques: Comparison

    Semen concentration measurement results (mean, SD, minimum and maximum) from 109 diluted boar sperm samples. Mean values differ significantly depending on measurement method ( a,b P < 0.0001).

    Journal: Scientific Reports

    Article Title: Comparison of NUCLEOCOUNTER, ANDROVISION with Leja chambers and the newly developed ANDROVISION eFlow for sperm concentration analysis in boars

    doi: 10.1038/s41598-022-16280-6

    Figure Lengend Snippet: Semen concentration measurement results (mean, SD, minimum and maximum) from 109 diluted boar sperm samples. Mean values differ significantly depending on measurement method ( a,b P < 0.0001).

    Article Snippet: To compare the concentration measurements of different methods, 109 diluted boar semen samples from a commercial AI center were measured for sperm concentration with the NUCLEOCOUNTER, ANDROVISION eFlow and ANDROVISION with disposable counting chambers (Leja, 4-chamber counting slides; 20 μm nominal chamber depth, Leja Products B.V., Nieuw-Vennep, The Netherlands) in a split sample design.

    Techniques: Concentration Assay